'Gender' in linguistics refers to nouns being masculine or feminine.
For example, most European languages have 'grammatical' gender in which all nouns (even objects) are masculine or feminine, often depending on their forms.
In Spanish, la mesa (the table) is a feminine noun (because it ends in an -a), and el suelo (the floor) is masculine (because it ends in -o). This affects the grammar, i.e. why it's la mesa and el suelo.
English (like Japanese) doesn't have gender, so the table and the floor are the same in grammatical terms.
However, of course, in all languages there are nouns that refer to things that are (biologically) male or female, like man, woman, boy, girl, but that's different from grammatical gender.