I use any number of variations, basically just going by whatever right given who I'm writing to, the nature of the communication (a request, asking a favor, an apology, etc.)
Just pulling up random emails from my sent box over the past few days:
以上、お手数をお掛けしますが、ご確認のほどよろしくお願いいたします。
それでは、引き続きよろしくお願いいたします。
ご迷惑をお掛けしますが、よろしくお願い申し上げます。
それでは、ご検討のほどよろしくお願いいたします。
今後とも何卒よろしくお願いいたします。
では、引き続きよろしくお願いします。
etc. etc.
Just from the above, you can probably gather a bit about the content of the email or the relationship with the recipient.
Even if it's not a request, per se, you really can't go wrong with a よろしくお願いします.
以上 isn't really required, and I typically only include it when I specifically want to (re-)call attention to what I've said above.
Just ending an email with 以上 only is very abrupt. I can see it in a brusque businesslike directive from a boss to subordinates, but as a freelancer corresponding directly with clients I wouldn't really have any occasion to close an email like that.