Hitoyoshi (人吉市, Hitoyoshi-shi) is a city in Kumamoto Prefecture, Japan. As of October 2016, the city has an estimated population of 33,461 and a population density of 160 persons per km2. The total area is 210.55 km2. It is famous for boat trips on the river, hot springs and former Hitoyoshi Castle. Aoi Aso Shrine, one of the National Treasures of Japan, is located in Hitoyoshi. It is bounded to the north, east, and west by towns of Kuma District, and to the south by Kagoshima Prefecture.